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| Registros recuperados: 27 | |
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| Cochennec, Nathalie; Hervio, Dominique; Panatier, B; Boulo, Viviane; Mialhe, Eric; Rogier, H; Grizel, Henri; Paolucci, F. |
| Currently recognition of molluscan diseases relies heavily upon light microscopic examination of stained histological sections of molluscan tissues Such histopathological methods are time consuming and relatively inefficient In the study of ongoing epizootics Alternatively, more effluent investigative tools are needed to accelerate disease recognition and quantification Such methods are needed to prevent control and eradicate molluscan diseases This paper reports the development of a direct sandwich enzyme-lmmunosorbent assay (ELISA) for the ascetosporan intrahemocytic parasite Bonamiao ostreae of the flat oyster Ostrea edulis. Bonamiasis is a commercially important epizootic disease of oysters A Bonamia-specific monoclonal antibody is employed in the... |
| Tipo: Text |
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| Ano: 1992 |
URL: http://archimer.ifremer.fr/doc/1992/publication-2765.pdf |
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| Grizel, Henri; Mialhe, Eric; Chagot, Dominique; Boulo, Viviane; Bachere, Evelyne. |
| Research on bonamiasis, an epizootic disease of the edible oyster Ostrea edu/is caused by the protozoan Bonamia ostreae, is discussed in relation to oyster fanning, research technology, epizootiology, and management. Morphological and infectious characteristics of the parasite are described. Recent progress in isolation and purification of the parasite have permitted investigations into host defense mechanisms, parasite infectivity, and the development of a mol1usc-pathogen mode!. |
| Tipo: Text |
Palavras-chave: Epizootic disease; Pathology; Protozoan; Bonamia ostreae; Oysters. |
| Ano: 1988 |
URL: http://archimer.ifremer.fr/doc/1988/publication-3120.pdf |
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| Le Gall, G; Bachere, Evelyne; Mialhe, Eric. |
| During a study of Rickettsiales-like organisms (RLO) in Pecten maximus gill, histological observation of free RLO in hemoclymph led us to consider interactions between the pathogen and the hemocytes. Firstly, the production of oxygen radicals by hemocytes was established using a luminol-enhanced chemiluminescence method with zymosan axs stimulant. The effects of serveral inhibitors of oxidative metabolism were investigated. The internalization of RLO into hemocytes was demonstrated by electron microscopy but was not related to chemiluminescent activity. The possible involvement of RLO acid phosphatase as the inhibitor of respiratory burst was assessed. |
| Tipo: Text |
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| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/publication-2759.pdf |
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| Le Gall, G; Mialhe, Eric; Chagot, D; Grizel, Henri. |
| Rickettsia-like microorganisms were described in gill endothelial cells during mass mortalities of Saint-Jacques scallops Pecten maximus in Saint-Brieuc Bay (North Brittany, France) during winter 1985-86. In order to evaluate the epizootiological significance of rickettsiosis of scallops, an analytical and descriptive epizootiological study was undertaken. The study included the geographic distribution, incidence and severity of the disease for several northern European and French scallop populations. Since the center of French scallop production is in Saint-Brieuc Bay, epizootiological investigations were concentrated in this area. Dissemination appeared to be by horizontal transmission. The pathogenic significance of the rickettsia-like organism in... |
| Tipo: Text |
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| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/publication-2758.pdf |
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| Kellner-cousin, Kristell; Le Gall, G; Despres, Béatrice; Kaghad, M; Legoux, P; Shire, D; Mialhe, Eric. |
| Genomic DNA fragments of a rickettsia-like organism (RLO) of Saint-Jacques scallop, Pecten maximus, were extracted from purified RLO and cloned. A DNA sequence of 1500 bp taken from one of the sequenced clones was coupled to horseradish peroxidase and the resulting probe was assayed for the diagnosis of RLO by nucleic acid hybridization. The specificity of the probe for RLO DNA was established from its lack of hybridization with scallop DNA. The sensitivity limit of the probe detected by enhanced chemiluminescence was determined to be around 500 ng of total RLO nucleic acids, equivalent to about 2.5 X 106 copies of RLO DNA. A pair of polymerase chain reaction (PCR) primers were synthesized that amplified a 1300 bp segment of the 1500 bp RLO fragment. After... |
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| Ano: 1993 |
URL: http://archimer.ifremer.fr/doc/1993/publication-2771.pdf |
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| Boulo, Viviane; Hervio, Dominique; Morvan, Annie; Bachere, Evelyne; Mialhe, Eric. |
| The chief importance of hemocytes as immune effectors in molluscs has led to develop researches for in vitro culture. The chemiluminescence technique has been used to estimate the phagocytosis capacity of Crassostrea gigas hemocytes according to different culture media and times. Sea water was used as a basic medium. Additives, such as antibiotics or glucose and buffers, such as Tris or Hepes, were tested as weIl as artificial sea water. Hemocyte chemiluminescence activities were recorded at t=o in relation to the medium composition. The highest values were observed when hemocytes were kept in sea water without any modification. Whichever medium, chemiluminescence activity was substantially decreased when hemocytes were previously kept in vitro for a few... |
| Tipo: Text |
Palavras-chave: Crassotrea gigas; Oyster; Bonamia ostreae; Pathogens; Molluscs; Hemocytes. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/acte-4115.PDF |
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| Hervio, Dominique; Chagot, Dominique; Godin, Pierre; Grizel, Henri; Mialhe, Eric. |
| Acid phosphatase activity was cytochemically detected at the ultrastructural level in the protozoan Bonamia ostreae, an intrahemocytic parasite of the flat oyster Ostrea edulis. Lead nitrate was used as capture agent for phosphate liberated during the hydrolysis of the subtrate (cytidine 5' monophosphate). Lead phosphate precipiation, indicative of enzyme activity, was located in membrane-bound organelles corresponding to the cytoplasmic inclusions known as "dense bodies". Acid phosphatase activity level and sodium L-tartrate inhibition were measured spectrophotometrically from a purified parasite suspension. The possible involvement of this enzyme in intracellular survival of B. ostrea is discussed. |
| Tipo: Text |
Palavras-chave: Acid phosphate activity; Ostrea edulis; Bonamia ostreae. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/publication-2755.pdf |
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| Hervio, Dominique; Chagot, D; Godin, P; Grizel, Henri; Mialhe, Eric. |
| Acid phosphatase activity was cytochemically detected at the ultrastructural level in the protozoan Bonamia ostreae, an intrahemocytic parasite of the flat oyster Ostrea edulis. Lead nitrate was used as capture agent for phosphate liberated during the hydrolysis of the substrate (cytidine 5' monophosphate). Lead phosphate precipitation, indicative of enzyme activity, was located in membrane-bound organelles corresponding to the cytoplasmic inclusions known as 'dense bodies'. Acid phosphatase activity level and sodium L-tartrate inhibition were measured spectrophotometrically from a purified parasite suspension. The possible involvement of this enzyme in intracellular survival of B. ostreae is discussed. |
| Tipo: Text |
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| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/00182/29311/28273.pdf |
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| Bachere, Evelyne; Hervio, Dominique; Mialhe, Eric. |
| Microbicidal phagocytic function and oxidative metabolism of the hemocytes of 2 oyster species, Ostrea edulis and Crassostrea gigas, were investigated using a luminol-enhanced chemiluminescence (technique. First, experimental parameters adapted to marine bivalve hemocytes were established on hemolymph pools of C. gigas in order to obtain qualitatively and quantitatively homogeneous samples and so to perform statistically comparable assays. The use of Modified Alsever Solution (allowed hemocytes to be kept non-aggregated and in a non-stimulated state until starting the assays. A number of aliquots of 2 X 10(5) hemocytes with an MAS final concentration of 3.5 % showed high chemiluminescent response after stimulation by zymosan particles A particle:hemocyte... |
| Tipo: Text |
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| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/publication-2761.pdf |
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| Rogier, Hervé; Hervio, Dominique; Boulo, Viviane; Clavies, Christine; Hervaud, Eliane; Bachere, Evelyne; Mialhe, Eric; Grizel, Henri; Pau, Bernard; Paolucci, Francis. |
| The protozoan Bonamia ostreae (Ascetospora), a paasite of the flat oyster Ostrea edulis, was purified by differential and isopycnic centrifugations. Mice of the strain Balb/c were immunized with purified parasites and hybridomas were prepared by fusion of immunized mouse splenocytes with the mouse myeloma cell line P3-X63-Ag8-653. From the fusion, 12 clones were isolated and saved. The resulting antibodies were characterized with a solid phase radioimmunoassay (RIA). Antibodies from 7 clones reacted only with B. ostreae and not with normal host tissue. Although some of the antibiodies identified. One of these antibodies (20B2-1B12) has an apparent association constant of ca 3 X 10(8) M-1 and bound to a number of different sites on the parasite. These... |
| Tipo: Text |
Palavras-chave: Monoclonal antibodies; Oyster; Ostrea edulis; Bonamia ostreae. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/publication-2756.pdf |
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| Robledo, Jaf; Boulo, Viviane; Mialhe, Eric; Despres, Béatrice; Figueras, Antonio. |
| Digestive glands of mussels Mytilus edulis from Brittany, France, infected with Marteilia sp. (Ascetospora) were used to purify the parasite. A modification of a previously used purification protocol increased purification efficiency, permitting sporangial primordia and sporangia of Marteilia sp. to be obtained. Mouse (Balb/c) monoclonal antibodies were generated against this parasite. From the fusion, 26 monoclonal antibodies against Marteilia sp. were obtained. Antibodies from 6 clones reacted only with Marteilia sp. cells and not with normal host tissues. Four of these antibodies (1/1-3, 3/1-1, 4/1-1 and 6/2-3) reacted with the sporangia wall and two with the spore cytoplasm (9/1-1 and 12/5-1). Antibodies cross-reacted with Marteilia refringens from... |
| Tipo: Text |
Palavras-chave: Marteilia sp.; Mytilus edulis; Monoclonal antibodies. |
| Ano: 1994 |
URL: http://archimer.ifremer.fr/doc/00196/30730/29404.pdf |
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| Mialhe, Eric. |
| Les productions d'invertébrés sont prépondérantes en aquaculture marine qui est économiquement très importate pour un grand nombre de pays, notamment de la zone Sud. Cette activité est confrontée à de nombreux problèmes des pathologie qui remettent en cause sa périnnité et son développment. Jusque dans les années quatre-vingt, les travaux relatifs à la pathologie des mollusques et des crevettes ont essentiellement porté sur la description des agents pathogènes. La création en 1985 par l'ISTPM/IFREMER d'un laboratoire pourvu de tous les équipements nécessaires aux techniques de pathologie a permis de mettre en place un programme de recherches orienté vers le contrôle des maladies. Une double stratégie de travail a été alors défine pour apporter à... |
| Tipo: Text |
Palavras-chave: Pathologie; Immmunologie; Génétique; Mollusque; Crevette; Diagnostic; Epidémiologie; Traitement; Sélection; Transformation génétique. |
| Ano: 1994 |
URL: http://archimer.ifremer.fr/doc/00443/55479/57009.pdf |
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| Registros recuperados: 27 | |
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